Journal: Contemporary Oncology

Article Title: Quercetin inhibits the activity and function of dendritic cells through the TLR4/IRAK4/NF-κB signalling pathway

doi: 10.5114/wo.2023.133741

Figure Lengend Snippet: Effect of quercetin (QUE) on the ratio of CD86- and CD11cpositive cells on the dendritic cells surface ( n = 3 per group, error are the mean ±SEM). The ratio of CD86- and CD11c-positive cells among the various groups, separately (A–D, F–I). Statistical graph of the ratio of CD86 and CD11c positive cells, separately (E, J). Lipopolysaccharide group compared with the normal control group. Lipopolysaccharide group compared with the QUE and IRAK4-IN-4 group *** p < 0.001 **** p < 0.0001 # p < 0.05 #### p < 0.0001 IRAK4 – interleukin-1 receptor-associated kinase 4, LPS – lipopolysaccharide, NC – normal control, QUE – quercetin

Article Snippet: Quercetin (Puxi, China); lipopolysaccharide (LPS) (Sigma-Aldrich, China); TLR4 (Beyotime, China); NF-κB p65 (CST, USA); IRAK4 (Abcam, USA); BCA kit (Thermo, USA); FITC anti-CD86 antibody and PE anti- CD11c antibody (BD Biosciences, USA); Cell Fixation Membrane Breaker (eBioscience, USA); SDS-PAGE electrophoresis system, Western blotting transfer system, ChemiDoc MP multifunctional chemiluminescence imaging system (Bio-Rad, USA); BD FACSCanto II flow cytometer (BD Bioscience USA); Enzyme labeller SpectraMax Paradigm MultiMode Microplate Reader (Molecular Devices, USA).

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